Silicon photonic microring resonators for the development of cancer protein biomarker assays

biosensor;silicon photonics;microring resonators;biomarker;blood serum analysis;cancer;assay;bioanalysis;multiplex sensors

Washburn, Adam

关键词: biosensor;    silicon photonics;    microring resonators;    biomarker;    blood serum analysis;    cancer;    assay;    bioanalysis;    multiplex sensors;   

Others  :  https://www.ideals.illinois.edu/bitstream/handle/2142/31162/Washburn_Adam.pdf?sequence=1&isAllowed=y

美国|英语

来源: The Illinois Digital Environment for Access to Learning and Scholarship

【 摘 要 】

Although often referred to as simply "cancer," uncontrolled cell growth and division in a human body is often the result of a variety of biochemical perturbations. Efforts to improve diagnosis and treatment of cancer patients are increasingly relying upon more detailed individual biochemical information—i.e. a "personalized medicine" approach. One method to ascertain individual biochemical conditions is by measuring the levels of certain proteins in the blood stream, commonly referred to as cancer biomarkers. Traditional quantitative methods for biomarker analysis have typically relied on a single biomarker to provide information. However, single biomarker analyses have proven to be woefully incomplete in the information content needed for a personalized medical diagnosis. Multiplexed biomarker panels have the potential to overcome the limitations of single biomarker analysis due to the greater information content present in a panel of biomarkers, but technologies to rapidly, accurately, and reproducibly make multiplexed measurements, especially in a quantitative manner, are as yet undeveloped. This doctoral dissertation presents the development of silicon photonic microring resonator technology in the Ryan Bailey research laboratory towards multiplexed cancer protein biomarker measurements. Microring resonator sensing technology relies upon changes in refractive index that induce a resonance shift in circular silicon waveguides. Taking advantage of standard semiconductor processing techniques and facilities, it has been possible to rapidly develop sensor chips with 32 active sensors with the potential to be scaled even higher. These chips have thus been developed for making multiplexed biomarker measurements.This dissertation represents the development of microring resonator technology from a novel technology to applied multiplex detection in cancer patient blood serum samples. Chapter 1 introduces more fully the topic of microring resonators, followed by a discussion of the basic physical characteristics, properties, and abilities of the sensors in Chapter 2. Chapter 3 introduces singleplex protein biomarker detection with multiplex detection in buffer following in Chapter 4. Multiplexed capture agent screening, a necessity for multiplexed immunoassays, is covered in Chapter 5 followed by the singleplex detection of a protein biomarker in human serum in Chapter 6. The final work of this project concludes with Chapter 7 which describes the methods used to make multiplex cancer biomarker measurements in human serum.