Biological behavior of Trypanosoma cruzi stocks obtained from the state of Amazonas, Western Brazilian Amazon, in mice

Revista da Sociedade Brasileira de Medicina Tropical

Wuelton Marcelo Monteiro²  Laylah Kelre Costa Magalhães²  Josué Costa Oliveira²  Jorge Augusto De Oliveira Guerra²  Henrique Silveira²  Luiz Carlos De Lima Ferreira²  Max Jean De Ornelas Toledo¹  Maria Das Graças Vale Barbosa² 

[1] ,Universidade do Estado do Amazonas Fundação de Medicina Tropical Dr. Heitor Vieira Dourado Programa de Pós-Graduação em Medicina TropicalManaus AM

关键词: Chagas disease;    Trypanosoma cruzi;    Virulence;    Pathogenicity;    Swiss mice;    Amazon;    Doença de Chagas;    Trypanosoma cruzi;    Virulência;    Patogenicidade;    Camundongos Swiss;    Amazônia;   

DOI  :  10.1590/S0037-86822012000200014

来源: SciELO

【 摘 要 】

INTRODUCTION: The biological diversity of circulating Trypanosoma cruzi stocks in the Amazon region most likely plays an important role in the peculiar clinic-epidemiological features of Chagas disease in this area. METHODS: Seven stocks of T. cruzi were recently isolated in the State of Amazonas, Brazil, from humans, wild mammals, and triatomines. They belonged to the TcI and Z3 genotypes and were biologically characterized in Swiss mice. Parasitological and histopathological parameters were determined. RESULTS: Four stocks did not promote patent parasitemia in mice. Three stocks produced low parasitemia, long pre-patent periods, and a patent period of 1 day or oscillating parasitemia. Maximum parasitemia ranged from 1,400 to 2,800 trypomastigotes/0.1mL blood. Mice inoculated with the T. cruzi stocks studied showed low positivity during fresh blood examinations, ranging from 0% to 28.6%. In hemoculture, positivity ranged from 0% to 100%. Heart tissue parasitism was observed in mice inoculated with stocks AM49 and AM61. Stock AM49 triggered a moderate inflammatory process in heart tissue. A mild inflammatory process was observed in heart tissue for stocks AM28, AM38, AM61, and AM69. An inflammatory process was frequently observed in skeletal muscle. Examinations of brain tissue revealed inflammatory foci and gliosis in mice inoculated with stock AM49. CONCLUSIONS: Biological and histopathological characterization allowed us to demonstrate the low infectivity and virulence of T. cruzi stocks isolated from the State of Amazonas.

【 授权许可】

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