Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model

Revista da Sociedade Brasileira de Medicina Tropical

César Gómez-hernández²  Karine Rezende-oliveira¹  Gabriel Antônio Nogueira Nascentes¹  Lara Rocha Batista²  Henrique Borges Kappel²  José Alejandro Martinez-ibarra¹  Francisco Trujillo Contreras¹  Eliane Lages-silva¹  Luis Eduardo Ramírez¹ 

[1] ,Universidade Federal do Triângulo Mineiro Departamento de Clínica Médica Programa de Pós-Graduação em Medicina Tropical e InfectologiaUberaba MG

关键词: Trypanosoma cruzi;    Mexico;    Biological characterization;    Genetic characterization;    Trypanosoma cruzi;    México;    Caracterização biológica;    Caracterização genética;   

DOI  :  10.1590/S0037-86822011005000058

来源: SciELO

【 摘 要 】

INTRODUCTION: For a long time, the importance of Chagas disease in Mexico, where many regarded it as an exotic malady, was questioned. Considering the great genetic diversity among isolates of Trypanosoma cruzi, the importance of this biological characterization, and the paucity of information on the clinical and biological aspects of Chagas disease in Mexico, this study aimed to identify the molecular and biological characterization of Trypanosoma cruzi isolates from different endemic areas of this country, especially of the State of Jalisco. METHODS: Eight Mexican Trypanosoma cruzi strains were biologically and genetically characterized (PCR specific for Trypanosoma cruzi, multiplex-PCR, amplification of space no transcript of the genes of the mini-exon, amplification of polymorphic regions of the mini-exon, classification by amplification of intergenic regions of the spliced leader genes, RAPD - (random amplified polymorphic DNA). RESULTS: Two profiles of parasitaemia were observed, patent (peak parasitaemia of 4.6×10(6) to 10(7) parasites/mL) and subpatent. In addition, all isolates were able to infect 100% of the animals. The isolates mainly displayed tropism for striated (cardiac and skeletal) muscle. PCR amplification of the mini-exon gene classified the eight strains as TcI. The RAPD technique revealed intraspecies variation among isolates, distinguishing strains isolated from humans and triatomines and according to geographic origin. CONCLUSIONS: The Mexican T. cruzi strains are myotrophic and belong to group TcI.

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