Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model

Revista da Sociedade Brasileira de Medicina Tropical

Nascentes, Gabriel Antônio Nogueira¹  Universidade Federal de Uberlândia, Ituiutaba¹  Batista, Lara Rocha¹  Kappel, Henrique Borges¹  Gómez-Hernández, César¹  Martinez-Ibarra, José Alejandro¹  Rezende-Oliveira, Karine¹  Ramírez, Luis Eduardo¹  Centro Univerisitario de los Altos, Jalisco, México¹  Instituto Federal do Triângulo Mineiro, Uberaba¹  Universidade Federal do Triângulo Mineiro, Uberaba¹  Trujillo Contreras, Francisco¹  Centro Universitário del Sur, Jalisco, México¹  Lages-Silva, Eliane¹ 

关键词: Trypanosoma cruzi;    Mexico;    Biological characterization;    Genetic characterization;   

DOI  :  10.1590/S0037-86822011005000058

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Medicina Tropical

【 摘 要 】

INTRODUCTION: For a long time, the importance of Chagas disease in Mexico, where many regarded it as an exotic malady, was questioned. Considering the great genetic diversity among isolates of Trypanosoma cruzi, the importance of this biological characterization, and the paucity of information on the clinical and biological aspects of Chagas disease in Mexico, this study aimed to identify the molecular and biological characterization of Trypanosoma cruzi isolates from different endemic areas of this country, especially of the State of Jalisco.METHODS: Eight Mexican Trypanosoma cruzi strains were biologically and genetically characterized (PCR specific for Trypanosoma cruzi, multiplex-PCR, amplification of space no transcript of the genes of the mini-exon, amplification of polymorphic regions of the mini-exon, classification by amplification of intergenic regions of the spliced leader genes, RAPD - (random amplified polymorphic DNA).RESULTS: Two profiles of parasitaemia were observed, patent (peak parasitaemia of 4.6×106 to 107 parasites/mL) and subpatent. In addition, all isolates were able to infect 100% of the animals. The isolates mainly displayed tropism for striated (cardiac and skeletal) muscle. PCR amplification of the mini-exon gene classified the eight strains as TcI. The RAPD technique revealed intraspecies variation among isolates, distinguishing strains isolated from humans and triatomines and according to geographic origin.CONCLUSIONS: The Mexican T. cruzi strains are myotrophic and belong to group TcI.

【 授权许可】

Unknown