Investigation on the possibility of spontaneous cure of mice infected with different strains of Trypanosoma cruzi

Revista do Instituto de Medicina Tropical de São Paulo

Juracy B. Magalhães¹  Sonia G. Andrade¹ 

关键词: Trypanosoma cruzi;    Spontaneous cure;    Chronic infection;    Murine model;    Cyclophosphamide;   

DOI  :  10.1590/S0036-46651994000600001

来源: SciELO

【 摘 要 】

Seventy Swiss mice chronically infected with different strains of Trypanosoma cruzi, with persistently negative parasitemia on routine blood examination were parasitologically investigated to find out whether spontaneous cure occurred. Duration of infection varied from 90 to 250 days in the initial phase of this investigation. Parasitological tests consisted of daily direct blood examination performed during at least 25 days, followed by xenodiagnosis and subinoculation of blood into newborn mice. Mice that persisted negative were treated with Cyclophosphamide with one dose of 250 mg/kg of body weight and then investigated by direct blood examination, xenodiagnosis and subinoculation. A second dose of 250 mg/kg b. w. was given to the persistently negative mice. With one single exception, all mice showed positive parasitological tests in the different stages of the present investigation and we conclude that spontaneous cure did not occur in this group, which is representative of the chronic infection with different strains of T cruzi.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License