Murine virus contaminant of Trypanosoma cruzi experimental infection

Revista do Instituto de Medicina Tropical de São Paulo

Humberto De A. Rangel²  Liana Verinaud²  Irineu J. B. Camargo²  Rovilson Gilioli¹  Julia K. Sakurada² 

[1] ,Institute of Biology Department of Microbiology and Immunology

关键词: Chagas' disease;    Trypanosoma cruzi;    Immune response;    Coronavirus;   

DOI  :  10.1590/S0036-46651994000500006

来源: SciELO

【 摘 要 】

The possibility that some virus contaminants could be altering host response to Trypanosoma cruzi experimental infection was investigated. Data obtained showed that CBA/J mice infected with stocks of parasite maintained in mice (Y1UEC) presented higher level of parasitemia and shorter survival times than those infected with a stock (Y1TC) which was also maintained in mice but had been previously passaged in cell culture. Mouse antibody production tests, performed with the filtered plasma of mice infected with Y1UEC, indicated the presence of mouse hepatitis virus (MHV) while no virus was detected when testing the plasma of Y1TC infected mice. Filtered plasma of Y1EUC infected mice was shown to contain a factor able to enhance the level of parasitemia and to reduce the mean survival time of mice challenged with 10(5) Y1TC. This factor, that could be serially passaged to naïve mice was shown to be a coronavirus by neutralization tests.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License