Crystallization and preliminary crystallographic analysis of trypanothione reductase from Trypanosoma cruzi, the causative agent of Chagas' disease

FEBS Letters

Krauth-Siegel, R.Luise¹  Jöst, Ingrid¹  Lantwin, Christina B.²  Kabsch, Wolfgang²  Pai, Emil F.³  Sticherling, Christian¹  Walsh, Christopher T.⁴ 

[1] Institut für Biochemie II, Universität Heidelberg, Im Neuenheimer Feld 328, D-6900 Heidelberg, Germany;Abteilung Biophysik, Max-Planck-Institut für Medizinische Forschung, Jahnstrasse 29, D-6900 Heidelberg, Germany;Departments of Biochemistry and Molecular and Medical Genetics, University of Toronto, 1 Kings's College Circle, Toronto, Ont., M5S 1A8, Canada;Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA

关键词: Trypanothione reductase;    Drug design;    Chagas' disease;    Flavoprotein;    Protein crystallography;    Trypanosoma cruzi;    GSH;    glutathione;    GSSG;    glutathione disulfide;    TR;    trypanothione reductase;    TS2;    trypanothione disulfide;    T(SH)2;    trypanothione;   

DOI  :  10.1016/0014-5793(93)81501-P

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Trypanothione reductase from Trypanosoma cruzi is the most promising target molecule for the rational design of a specific drug against Chagas' disease. The recombinant protein was purified in a single Chromatographie step and crystallized. Two crystal forms suitable for X-ray diffraction analysis were obtained. Tetragonal crystals (a = b = 87.4 Å, c = 152.3 Å) were grown from 30% polyethylene glycol (average M _r = 8,000) in the presence of 0.2% β-n-octylglucoside (space group either P4₂ with one dimer or P4₂22 with one monomer in the asymmetric unit). Monoclinic crystals (space group P2, a = 136.3 Å, b = 91.1 Å, c = 126.0 Å, β = 94°) were grown from 1.2 M sodium citrate in the presence of 2% octanoyl-N-methyl-glucamide. They contain two dimers of the enzyme in the asymmetric unit; both crystal forms diffract to 3 Å resolution.

【 授权许可】

Unknown