Evaluation of different immunization protocols with P. brasiliensis antigens in Guinea pigs

Revista do Instituto de Medicina Tropical de São Paulo

Amélia Kamegasawa²  Maria Tereza Rezkallah Iwasso¹  Rosa Viero¹  Marcello Franco¹ 

[1],UNESP Faculty of Medicine Department of OphthalmologyBotucatu São Paulo ,Brasil

关键词: Paracoccidioides brasiliensis antigens;    Guinea pigs;    Immunization protocols;   

DOI  :  10.1590/S0036-46651992000300011

来源: SciELO

【 摘 要 】

The objective of the present study was to develop an efficient and reproducible protocol of immunization of guinea pigs with P. brasiliensis antigens as an animal model for future studies of protective immunity mechanisms. We tested three different antigens (particulate, soluble and combined) and six protocols in the presence and absence of Freund's complete adjuvant and with different numbers of immunizing doses and variable lenght of time between the last immunizing dose and challenge. The efficacy of the immunizing protocol was evaluated by measuring the humoral and cellular anti-P. brasiliensis immune response of the animals, using immuno-diffusion, skin test and macrophage migration inhibition test. It was observed that: 1. Three immunizing doses of the antigens induced a more marked response than two doses; 2. The highest immune response was obtained with the use of Freund's complete adjuvant; 3. Animals challenged a long time (week 6) after the last immunizing dose showed good anti-P. brasiliensis immune response; 4. The particulate antigen induced the lowest immune response. The soluble and the combined antigens were equally efficient in raising good humoral and cellular anti-P. brasiliensis immune response

【 授权许可】

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