In vitro action of some disinfectants on Paracoccidioides brasiliensis yeast forms

Revista do Instituto de Medicina Tropical de São Paulo

M. A. Shikanai-yasuda²  R. T. Taguchi¹  M. K. Sato¹  N. T. Melo¹  C. M. Assis¹  R. C. Nigro¹  E. E. Camargo¹  C. S. Lacaz¹  V. Amato Neto¹  A. Sesso¹ 

[1] ,Hospital das Clínicas Laboratório de Imunologia Médica Depto. de Doenças Infecciosas e Parasitárias

关键词: Paracoccidioides brasiliensis;    Disinfectants;    Fluorescein diacetate-ethidium bromide;    Yeast to mycelial germination;    Radiometric method;    Sodium hypochlorite;   

DOI  :  10.1590/S0036-46651991000100008

来源: SciELO

【 摘 要 】

The fungicidal action of sodium hypochlorite (0.3, 1, 2.5, 5 and 10%); formaldehyde (2, 5, and 10%); and ethyl alcohol (70%) on yeast forms of Paracoccidioides brasiliensis Pb 18 and a newly-isolated Goiana strain was described. Contact between the fungus and the disinfectants was maintained for 1, 2, 24, 48 and 72 hours at room temperature. Viability was evaluated by the fluorescein diacetate-ethidium bromide treatment, culture in solid and liquid media (36ºC and 26ºC); yeast to mycelial germination at room temperature; and radiometric study of metabolic activity. All concentrations of disinfectants were found to be effective in inactivating Pb 18 and Goiana strains, except for the 1-hour contact with 2% formaldehyde, in which fluorescein diacetate-ethidium bromide treatment was found to reveal 40 and 27% of viable cells, respectively. The yeast to mycelial germination method was considered to reveal faster and similar results as compared to culture in solid and liquid media.

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