Inactivation of various influenza strains to model avian influenza (Bird Flu) with various disinfectant chemistries.

Oberst, R. D. ; Bieker, Jill Marie ; Souza, Caroline Ann

Sandia National Laboratories

关键词: Pathogenic Microorganisms.;    Rna;    Disinfectants;    Ethanol;    Influenza-Prevention &;   

DOI  :  10.2172/877140
RP-ID  :  SAND2005-7633
RP-ID  :  AC04-94AL85000
RP-ID  :  877140

美国|英语

来源: UNT Digital Library

【 摘 要 】

Due to the grave public health implications and economic impact possible with the emergence of the highly pathogenic avian influenza A isolate, H5N1, currently circulating in Asia we have evaluated the efficacy of various disinfectant chemistries against surrogate influenza A strains. Chemistries included in the tests were household bleach, ethanol, Virkon S{reg_sign}, and a modified version of the Sandia National Laboratories developed DF-200 (DF-200d, a diluted version of the standard DF-200 formulation). Validation efforts followed EPA guidelines for evaluating chemical disinfectants against viruses. The efficacy of the various chemistries was determined by infectivity, quantitative RNA, and qualitative protein assays. Additionally, organic challenges using combined poultry feces and litter material were included in the experiments to simulate environments in which decontamination and remediation will likely occur. In all assays, 10% bleach and Sandia DF-200d were the most efficacious treatments against two influenza A isolates (mammalian and avian) as they provided the most rapid and complete inactivation of influenza A viruses.