Preparation of Modified RNA Sequences for Biological Research

CHIMIA

卷:59

关键词: Ligation;    Nucleic acids;    Organic synthesis;    Phosphoramidites;    Rna;   

DOI  :  10.2533/000942905777675633

来源: DOAJ

【 摘 要 】

Our group is working on the development of reliable access to biologically relevant, long, and modified RNA sequences. Here, we briefly present the chemical synthesis of such sequences with 2?-O-triisopropylsilyloxymethyl (= 2?-O-tom) protected ribonucleoside phosphoramidites, their template-directed enzymatic ligation, and some examples of modified nucleotides, designed to promote structural and biological studies.

【 授权许可】

Unknown