Evaluation of the photodynamic activity of Xanthene Dyes on Artemia salina described by chemometric approaches

Anais da Academia Brasileira de Ciências

Diogo S. Pellosi¹  Vagner R. Batistela¹  Vagner R. De Souza¹  Ieda S. Scarminio¹  Wilker Caetano¹  Noboru Hioka¹ 

关键词: Artemia salina;    factorial design;    photodynamic therapy;    bioassay;    xanthenes;    Artêmia salina;    planejamento fatorial;    terapia fotodinâmica;    bioensaio;    xantenos;   

DOI  :  10.1590/0001-3765201395412

来源: SciELO

【 摘 要 】

The development of drugs for photodynamic therapy (PDT) is an important area of research due to their growing use in medical applications. Therefore, it is important to develop new bioassay methods for PDT photosensitizers that are inexpensive, easy to handle and highly sensitive to environmental conditions. Xanthene dyes (fluorescein, rose bengal B, erythrosine B and eosin Y) with LED light sources were investigated using Artemia salina as a bioindicator of photodynamic activity. In this study, three factors were investigated: (i) photosensitizers concentration, (ii) the LED irradiation time and (iii) the waiting time between the addition of the photosensitizers and the beginning of the irradiation. To analyze the photo-killing of A. salina, it was employed a 23 full factorial design. The death of A. salina was related to dye structure and the interaction between the irradiation time and the photosensitizers concentration. About 60% of crustaceans death was obtained using rose bengal B, which presentes the highest quantum yield of singlet oxygen due to the number of iodide substituents in the xanthenes ring. The proposed bioassay using A. salina, xanthene dyes and LED irradiation was found suitable for quantitative PDT drug evaluation.

【 授权许可】

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