Electrosprayed naringin-loaded microsphere/SAIB hybrid depots enhance bone formation in a mouse calvarial defect model

Drug Delivery

Xiaohong Wu¹  Huthayfa N.S Almassri¹  Qiongyue Zhang¹  Xue Yang¹  Dan Zhang¹  Yihui Ma¹  Mingsheng Chen¹ 

[1] Department of Prosthodontics, Stomatological Hospital of Chongqing Medical University, Chongqing, China;Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing, China;Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing, Chin;

关键词: Burst release;    osteogenesis;    sucrose acetate isobutyrate;    naringin;    microsphere;   

DOI  :  10.1080/10717544.2019.1568620

来源: publisher

【 摘 要 】

The burst release of active osteogenic factors, which is not beneficial to osteogenesis, is commonly encountered in bone tissue engineering. The aims of this study were to prepare naringin-loaded microsphere/sucrose acetate isobutyrate (Ng-m-SAIB) hybrid depots, reduce the burst release of naringin (Ng), and improve osteogenesis. The morphology and size distributions of electrosprayed Ng-microspheres were characterized by scanning electron microscopy (SEM). The Ng-microspheres and Ng-m-SAIB depots were characterized by Fourier transform infrared spectroscopy (FTIR) and in vitro release studies. In vitro osteoblast-microsphere interactions and in vivo osteogenesis were assessed after implantation of Ng-m-SAIB depots. The addition of sucrose acetate isobutyrate (SAIB) to monodisperse Ng-microspheres did not cause a change in the chemical structure. The performances of the microspheres in osteoblast-microsphere interactions were better when the naringin content was 4% than when it was at 2% and 6%. On the first day following the loading of Ng-microspheres (2%, 4%, and 6%) into SAIB depots, the burst release was reduced dramatically from 70.9% to 6.3%, 73.1% to 7.2%, and 73.9% to 9.9%, respectively. In addition, after 8 weeks, the new bone formation rate in the calvarial defects of SD rats receiving Ng-m-SAIB was 53.1% compared to 21.2% for the control group and 16.1% for the microsphere-SAIB group. These results demonstrated that Ng-m-SAIB hybrid depots may have promise in bone regeneration applications.

【 授权许可】

CC BY