Oxidative stress and DNA damage induced by spinosad exposure in Spodoptera frugiperda Sf9 cells

Food and Agricultural Immunology

Jufang Gao¹  Yang Zhang²  Liming Tao²  Wenping Xu²  Mingjun Yang³ 

[1] College of Life and Environmental Sciences, Shanghai Normal University, Shanghai, People’s Republic of Chin;Shanghai Key Lab of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai, People’s Republic of Chin;State Key Labs of Family Planning Devices, Shanghai Institute of Planned Parenthood Research, Shanghai, People’s Republic of Chin;

关键词: Oxidative stress;    DNA damage;    spinosad;    sf9 cells;   

DOI  :  10.1080/09540105.2017.1364708

来源: publisher

【 摘 要 】

Spinosad, a neurotoxic insecticide, is widely used for crop protection. In order to elucidate the effects of spinosad on oxidative stress and genotoxicity in Sf9 cells, the levels of lipid peroxidation, the activity of antioxidative enzymes, and DNA damage were measured. The results showed that spinosad caused a time-dependent increase in the formation of malondialdehyde and decrease in the activity of superoxide dismutase and catalase. Further studies confirmed that spinosad induced 8-oxoguanine accumulation in Sf9 cells, which is accompanied by increased expression of DNA repair enzymes (OGG1 and MTH1). The neutral comet assay revealed that spinosad induced significant time-related increases of DNA double-strand breaks in Sf9 cells. Our results indicate that spinosad effectively induced oxidative stress and DNA damage in Sf9 cells.

【 授权许可】

CC BY