Permeation-enhancing effects and mechanisms of O-acylterpineol on isosorbide dinitrate: mechanistic insights based on ATR-FTIR spectroscopy, molecular modeling, and CLSM images

Drug Delivery

Jian Wang¹  Chunyan Wang²  Tianzhe Chu³  Yan Li⁴  Ligang Zhao⁵  Linlin Zhao⁵ 

[1] Department of Pharmaceutical Sciences, Shenyang Pharmaceutical University, Shenyang, China;Department of Pharmacy, Tangshan Maternal and Child Health Hospital, Tangshan, China;School of Pharmacy, North China University of Science and Technology, Tangshan, China;School of Pharmacy, North China University of Science and Technology, Tangshan, China;Department of Pharmacy, Tangshan Maternal and Child Health Hospital, Tangshan, China;School of Pharmacy, North China University of Science and Technology, Tangshan, China;Tangshan Key Laboratory of Novel Preparations and Drug Release Technology, Tangshan, Chin;

关键词: O;    penetration enhancers;    fatty acid;    transdermal drug delivery;    penetraion mechanism;   

DOI  :  10.1080/10717544.2018.1561764

来源: publisher

【 摘 要 】

The present study aimed to evaluate the penetration activity of O-acylterpineol derivatives both in vitro and in vivo, and to investigate the enhancing mechanism of O-acylterpineol derivatives which were synthesized by α-terpineol and fatty acid. The promoting activities on the isosorbide dinitrate patch were tested across full thickness rabbit skin both in vitro and in vivo. In order to elucidate the permeation mechanism, attenuated total reflection Fourier transform infrared spectroscopy, molecular modeling, and confocal laser scanning microscopy were introduced to investigate the regulation of enhancers in the skin permeability and biophysical properties. With in vitro cytotoxicity test and in vivo erythema model, the skin irritation of enhancers was also evaluated. Permeation studies showed 2-(4-methylcyclohex-3-en-l-yl) propan-2-yl tetradecanoate produced the obvious enhancement activity for ISDN both in vitro and in vivo from patches. These results were supported by ATR-FTIR, molecular modeling, and CLSM studies which revealed that O-acylterpineol could decrease the order of the alkyl chains in the skin lipids. Additionally, it was found that TER-C14 produced a relatively low skin irritation, compared with the TER which was assumed to be a safe compound. The present research suggested that some newly designed acylterpineol derivatives are shown to be suitable permeation enhancers for transdermal drug delivery, and the chain length of C14 seem to be safe and more favorable for the penetration of ISDN from DIA patches.

【 授权许可】

CC BY