The effect of bergamot polyphenolic fraction on lipid transfer protein system and vascular oxidative stress in a rat model of hyperlipemia

Lipids in Health and Disease

Salvatore Ragusa¹  Roberta Macrì²  Vincenzo Musolino²  Ernesto Palma²  Federica Scarano²  Francesca Bosco²  Cristina Carresi²  Jessica Maiuolo²  Miriam Scicchitano²  Stefano Ruga²  Micaela Gliozzi²  Sara Paone²  Maria Caterina Zito²  Carmen Colica²  Rocco Mollace²  Saverio Nucera²  Carolina Muscoli³  Vincenzo Mollace³ 

[1]0000 0001 2168 2547, grid.411489.1, Department of Health Sciences, University “Magna Graecia” of Catanzaro, Catanzaro, Italy

[2]0000 0001 2168 2547, grid.411489.1, Institute of Research for Food Safety & Health (IRC-FSH), Department of Health Sciences, University “Magna Graecia” of Catanzaro, Viale Europa, Loc. Germaneto, 88100, Catanzaro, Italy

[3]Nutramed S.c.a.r.l., Complesso Ninì Barbieri, 88021, Catanzaro, Roccelletta di Borgia, Italy

[4]0000 0001 2168 2547, grid.411489.1, Institute of Research for Food Safety & Health (IRC-FSH), Department of Health Sciences, University “Magna Graecia” of Catanzaro, Viale Europa, Loc. Germaneto, 88100, Catanzaro, Italy

[5]grid.414603.4, San Raffaele IRCCS Pisana, Rome, Italy

关键词: Bergamot polyphenolic fraction;    Lipid transfer protein system;    Hyperlipidaemia;    Oxidative stress;   

DOI  :  10.1186/s12944-019-1061-0

来源: publisher

【 摘 要 】

BackgroundExperimental and epidemiological studies show that bergamot polyphenolic fraction (BPF) ameliorates the serum lipemic profile, normalizes blood pressure and improves non alcoholic fatty liver disease in patients suffering from metabolic syndrome. Despite this evidence, the molecular mechanisms responsible for these beneficial effects remain unclear. The aim of our study is to clarify the effects of BPF on the lipoprotein assembly and to identify oxidative stress biomarkers correlating hyperlipidaemia and BPF-induced metabolic changes.MethodsMale Wistar rats (180–200 g) were randomly assigned to receive a standard diet, a hypercholesterolemic diet or a hypercholesterolemic diet+BPF (20 mg/Kg/rat daily, gavage), respectively, for 90 days. Total cholesterol (tChol), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), triglycerides (TG) and fasting plasma glucose were evaluated at the baseline as well as at the end of the treatment. To assess the effect of BPF on the Lipid Transfer Protein System, detection of ACAT, LCAT, CETP, PON1, Apo A1 and Apo B have also been carried out. Finally, the lipid peroxidation biomarker (TBARS) and oxyLDL were also measured.ResultsBPF prevented tChol, LDL-C, TG and fasting plasma glucose enhancement and improved HDL-C. Treatment of hyperlipæmic rats with BPF significantly restored altered the serum concentration of lipemic biomarkers and the activity of ACAT, LCAT, CETP and PON1, an effect accompanied by the concomitant normalization of Apo A1 and APO B levels. In addition, TBARS levels were reduced significantly by the treatment with BPF.ConclusionsBPF prevents diet-induced alteration of the lipid profile in rats, counteracting oxidative stress and improving the dysregulation of the Lipid Transfer Protein System. These data add new insights into the molecular mechanisms underlying the beneficial role of BPF in the therapy of hyperlipidaemia, thus suggesting a novel approach in the prevention of cardiovascular disease.

【 授权许可】

CC BY