Kin discrimination in social yeast is mediated by cell surface receptors of the Flo11 adhesin family

eLife

Timo Kraushaar¹  Lars Oliver Essen²  Daniel J Müller³  Rajib Schubert³  Stefan Brückner⁴  Daniel Hoffmann⁴  Hans-Ulrich Mösch⁵  Eric Jelli⁶  Raimo Hartmann⁶  Knut Drescher⁶ 

[1] Department of Biochemistry, Philipps-Universität Marburg, Marburg, Germany;Department of Biochemistry, Philipps-Universität Marburg, Marburg, Germany;LOEWE Center for Synthetic Microbiology, Philipps-Universität Marburg, Marburg, Germany;Department of Biosystems Science and Engineering, ETH Zürich, Basel, Switzerland;Department of Genetics, Philipps-Universität Marburg, Marburg, Germany;Department of Genetics, Philipps-Universität Marburg, Marburg, Germany;LOEWE Center for Synthetic Microbiology, Philipps-Universität Marburg, Marburg, Germany;Max Planck Institute for Terrestrial Microbiology, Marburg, Germany;

关键词: kin discrimination;    cell adhesion;    yeast;    biofilm;    Flo11 flocculin;    S. cerevisiae;   

DOI  :  10.7554/eLife.55587

来源: publisher

【 摘 要 】

Microorganisms have evolved specific cell surface molecules that enable discrimination between cells from the same and from a different kind. Here, we investigate the role of Flo11-type cell surface adhesins from social yeasts in kin discrimination. We measure the adhesion forces mediated by Flo11A-type domains using single-cell force spectroscopy, quantify Flo11A-based cell aggregation in populations and determine the Flo11A-dependent segregation of competing yeast strains in biofilms. We find that Flo11A domains from diverse yeast species confer remarkably strong adhesion forces by establishing homotypic interactions between single cells, leading to efficient cell aggregation and biofilm formation in homogenous populations. Heterotypic interactions between Flo11A domains from different yeast species or Saccharomyces cerevisiae strains confer weak adhesive forces and lead to efficient strain segregation in heterogenous populations, indicating that in social yeasts Flo11A-mediated cell adhesion is a major mechanism for kin discrimination at species and sub-species levels. These findings, together with our structure and mutation analysis of selected Flo11A domains, provide a rationale of how cell surface receptors have evolved in microorganisms to mediate kin discrimination.

【 授权许可】

CC BY