Cross-Reactive Sensor Array for Metal Ion Sensing Based on Fluorescent SAMs

Sensors

Lourdes Basabe-Desmonts¹  Frederieke van der Baan²  Rebecca S. Zimmerman²  David N. Reinhoudt² 

[1] id="af1-sensors-07-01731">Department of Supramolecular Chemistry and Technology, MESA⁺ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherland

关键词: High throughput;    fluorescence;    SAMs;    microtiter plate;    differential sensing;    sensor array;    metal ions;   

DOI  :  10.3390/s7091731

来源: mdpi

【 摘 要 】

Fluorescent self assembled monolayers (SAMs) on glass were previously developed in our group as new sensing materials for metal ions. These fluorescent SAMs are comprised by fluorophores and small molecules sequentially deposited on a monolayer on glass. The preorganization provided by the surface avoids the need for complex receptor design, allowing for a combinatorial approach to sensing systems based on small molecules. Now we show the fabrication of an effective microarray for the screening of metal ions and the properties of the sensing SAMs. A collection of fluorescent sensing SAMs was generated by combinatorial methods and immobilized on the glass surfaces of a custom-made 140 well microtiter-plate. The resulting libraries are easily measured and show varied responses to a series cations such as Cu²⁺, Co²⁺, Pb²⁺, Ca²⁺ and Zn²⁺. These surfaces are not designed to complex selectively a unique analyte but rather they are intended to produce fingerprint type responses to a range of analytes by less specific interactions. The unselective responses of the library to the presence of different cations generate a characteristic pattern for each analyte, a “finger print” response.

【 授权许可】

Unknown   
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