Marine Drugs | |
Chemopreventive Effects of Sarcotriol on Ultraviolet B-induced Skin Tumor Development in SKH-1 Hairless Mice | |
Vipra Kundoor2  Xiaoying Zhang2  Ajay Bommareddy2  Sherief Khalifa1  Hesham Fahmy2  | |
[1] Department of Pharmacognosy, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt;Department of Pharmaceutical Sciences, South Dakota State University, Box 2202C, Brookings, SD 57007, USA. Tel.: 605-688-4243, Fax 605-688-5993. | |
关键词:
Cancer chemoprevention;
Skin cancer;
Cembranoids;
Sarcophine;
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DOI : 10.3390/md504197 | |
来源: mdpi | |
【 摘 要 】
Sarcotriol (ST) has been shown to be chemopreventive on 7,12-dimethyl-benz(a)anthracene (DMBA) initiated and 12-O-tetradecanoylphorbol-13-acetate (TPA)-promoted skin tumor development in CD-1 mice in recent studies from our laboratory. The objective of this study was to determine the chemopreventive effects of ST on ultraviolet B (UVB)-induced skin tumor development in female SKH-1 hairless mice, an experimental model relevant to human skin cancer development, and its possible mechanisms of action. Female SKH-1 mice were divided into two groups: Control and ST treated. Control was topically treated with 100 μL acetone and ST treated group administered with 30 μg ST in 100 μL acetone one hour before UVB exposure. For UVB-induced tumorigenesis, carcinogenesis was initiated and promoted by UVB (180 mJ/cm2). Group weights and tumor counts were taken once every week. After 30 weeks, mice were sacrificed and dorsal skin samples were collected. The proteins from the skin sample were further used for SDS-PAGE and Western blotting using specific antibodies against caspase-3, caspase-8, caspase-9 and p53. Tumor multiplicity was found 19.6, 5.2 in the control and ST treated groups respectively. Caspase-3, -8, -9 and p53 were significantly (
【 授权许可】
CC BY
© 2007 by MDPI
【 预 览 】
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RO202003190058658ZK.pdf | 195KB | download |