Surface-enhanced Raman Spectral Measurements of 5-Fluorouracil in Saliva

Molecules

Stuart Farquharson¹  Alan Gift¹  Chetan Shende¹  Frank Inscore¹  Beth Ordway²  Carl Farquharson² 

[1] Real-Time Analyzers, Inc., Middletown, CT 06457, USA; E-mails:;Yale University, New Haven CT 06520, USA

关键词: 5-FU;    Chemotherapy;    Drug analysis;    Saliva analysis;    Raman;    SERS;   

DOI  :  10.3390/molecules13102608

来源: mdpi

【 摘 要 】

The ability of surface-enhanced Raman spectroscopy (SERS) to measure 5-fluorouracil (5-FU) in saliva is presented. The approach is based on the capacity of Raman spectroscopy to provide a unique spectral signature for virtually every chemical, and the ability of SERS to provide μg/mL sensitivity. A simple sampling method, that employed 1-mm glass capillaries filled with silver-doped sol-gels, was developed to isolate 5-FU from potential interfering chemical components of saliva and simultaneously provide SERS-activity. The method involved treating a 1 mL saliva sample with 1 mL of acetic acid, drawing 10 μL of sample into a SERS-active capillary by syringe, and then measuring the SER spectrum. Quality SER spectra were obtained for samples containing as little as 2 μg of 5-FU in 1 mL saliva. The entire process, the acid pretreatment, extraction and spectral measurement, took less than 5 minutes. The SERS of 5-fluorouridine and 5-fluoro-2’-deoxyuridine, two major metabolites of 5-FU, were also measured and shown to have unique spectral peaks. These measurements suggest that disposable SERS-active capillaries could be used to measure 5-FU and metabolite concentrations in chemotherapy patient saliva, thereby providing metabolic data that would allow regulating dosage. Tentative vibrational mode assignments for 5-FU and its metabolites are also given.

【 授权许可】

CC BY   
© 2008 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.