A New Method for the Determination of Nucleic Acid Using an Eu3+– nicotinic Acid Complex as a Resonance Light Scattering Probe

Molecules

Meng Guo²  Lin-Tong Wang²  Xia Wu¹  Wei Xu¹ 

[1] Key Laboratory of Colloid and Interface Chemistry (Shandong University), Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, P.R. China;Microscale Science Institute, Department of Chemistry, Weifang University, Weifang 261061, P.R. China. E-mail:

关键词: Nucleic acid;    Nicotinic acid;    Eu³⁺;    Resonance light scattering.;   

DOI  :  10.3390/molecules14010010

来源: mdpi

【 摘 要 】

This study found that in Tris-HCl buffer, the resonance light scattering (RLS) intensity of the Eu³⁺-nicotinic acid system can be greatly enhanced by nucleic acids and the enhanced intensity is proportional to the concentration of nucleic acid in the range of 7×10^-8-1×10^-5 g∙mL^-1 for fsDNA, and its detection limit is 2×10^-8 g∙mL^-1. Based on this, a new method for the determination of nucleic acids is proposed. Synthetic and actual samples are determined satisfactorily. The interaction mechanism is also studied. It is thought that nucleic acid can bind with the Eu³⁺-nicotinic acid complex through electrostatic attraction and thus form a large Eu³⁺-nicotinic acid-nucleic acid complex.

【 授权许可】

CC BY   
© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.