Fluorescence spectral characteristics of novel asymmetric monomethine cyanine dyes in nucleic acid solutions

FEBS Letters

Sabnis, Ram W¹  Ivanov, Ivan G³  Deligeorgiev, Todor G⁴  Maximova, Vera A³  Timcheva, Iliana I²  Gadjev, Nikolai I⁴ 

[1] Brewer Science Inc., P.O. Box GG, Rolla, MO 65401, USA;Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria;Institute of Molecular Biology, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria;Department of Organic Chemical Technology, Faculty of Chemistry, University of Sofia, 1126 Sofia, Bulgaria

关键词: Fluorescence;    Fluorescent dye;    Fluorescent label;    Intercalating agent;    Nucleic acid;    DNA;    RNA;    ds;    double stranded;    ss;    single stranded;    EtBr;    ethidium bromide;   

DOI  :  10.1016/S0014-5793(97)00171-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Six new asymmetric monomethine cyanine dyes have been synthesized and their fluorescence characteristics in the presence of nucleic acids studied. The new dyes have no fluorescence of their own in water solutions upon excitation at 480 nm but they become strongly fluorescent in the presence of nucleic acids. The fluorescence maxima of the investigated dyes are found at 525–545 nm when bound to dsDNA and around 600 nm upon binding to RNA and ssDNA. Fluorescence quenching studies with increasing concentrations of NaCl indicate that the cyanine dyes have a mixed (intercalating and groove binding) type of interaction with dsDNA. © 1997 Federation of European Biochemical Societies.

【 授权许可】

Unknown