Polarity Probing Two-Photon Fluorophores Based on [2.2]Paracyclophane

Bulletin of the Korean chemical society

Youngeup Jin¹  Han Young Woo¹  Dmitry Korystov¹  Hongsuk Suh¹ 

关键词: Two-photon absorption;    Bioimaging;    Two-photon fluorophore;    Paracyclophane;    Fluorescent label;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

A series of tetra donor substituted [2.2]paracyclophane-based two-photon absorption (TPA) fluorophores were synthesized in neutral and cationic forms. The imaging activity of overall set of fluorophores was studied by the two-photon induced fluorescence (TPIF) method in a range of solvents. We also measured a clear progression toward a longer photoluminescence lifetime with increasing solvent polarity (intrinsic photoluminescence lifetime, ンi: ~2 ns in toluene �? 12-16 ns in water). The paracyclophane fluorophores with this unique property can be utilized as an optical polarity probe for the biomolecular substrates. The combined measurement of the two-photon fluorescence microscopy (TPM) cell image and TPIF lifetime can give us a better understanding of the biological processes and local environments in the cells.

【 授权许可】

Unknown