Micro-Pattern Guided Adhesion of Osteoblasts on Diamond Surfaces

Sensors

Bohuslav Rezek¹  Lenka Michalíková¹  Egor Ukraintsev¹  Alexander Kromka¹ 

[1] Institute of Physics, Academy of Sciences of the Czech Republic, Cukrovarnicka 10, Prague 6, Czech Republic; E-Mail:

关键词: cell adhesion;    proteins;    diamond;    atomic force microscopy;    biotechnology;    tissue engineering;    biosensors;    osteoblasts;   

DOI  :  10.3390/s90503549

来源: mdpi

【 摘 要 】

Microscopic chemical patterning of diamond surfaces by hydrogen and oxygen surface atoms is used for self-assembly of human osteoblastic cells into micro-arrays. The cell adhesion and assembly is further controlled by concentration of cells (2,500-10,000 cells/cm²) and fetal bovine serum (0-15%). The cells are characterized by fluorescence microscopy of actin fibers and nuclei. The serum protein adsorption is studied by atomic force microscopy (AFM). The cells are arranged selectively on O-terminated patterns into 30-200 μm wide arrays. Higher cell concentrations allow colonization of unfavorable H-terminated regions due to mutual cell communication. There is no cell selectivity without the proteins in the medium. Based on the AFM, the proteins are present on both H- and O-terminated surfaces. Pronounced differences in their thickness, surface roughness, morphology, and phase images indicate different conformation of the proteins and explain the cell selectivity.

【 授权许可】

CC BY   
© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.