Evaluation of the In Vitro Biocompatibility of PEDOT:Nafion Coatings

Nanomaterials

Sonia Guzzo¹  Stefano Carli¹  Mauro Murgia¹  Michele Bianchi¹  Alice Lunghi¹  Barbara Pavan¹ 

[1] Center for Translational Neurophysiology, Istituto Italiano di Tecnologia, 44121 Ferrara, Italy;

关键词: conducting polymers;    organic bioelectronics;    surface coating;    cell adhesion;    cell proliferation;    atomic force microscopy;   

DOI  :  10.3390/nano11082022

来源: DOAJ

【 摘 要 】

Poly(3,4-ethylenedioxythiophene)-Nafion (PEDOT:Nafion) is emerging as a promising alternative to PEDOT-polystyrene sulfonate (PEDOT:PSS) in organic bioelectronics. However, the biocompatibility of PEDOT:Nafion has not been investigated to date, limiting its deployment toward in vivo applications such as neural recording and stimulation. In the present study, the in vitro cytotoxicity of PEDOT:Nafion coatings, obtained by a water-based PEDOT:Nafion formulation, was evaluated using a primary cell culture of rat fibroblasts. The surface of PEDOT:Nafion coating was characterized by Atomic Force Microscopy (AFM) and water contact angle measurements. Fibroblasts adhesion and morphology was investigated by scanning electron microscopy (SEM) and AFM measurements. Cell proliferation was assessed by fluorescence microscopy, while cell viability was quantified by 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT), lactate dehydrogenase (LDH) and neutral red assays. The results showed that PEDOT:Nafion coatings obtained by the water dispersion were not cytotoxic, making the latter a reliable alternative to PEDOT:PSS dispersion, especially in terms of chronic in vivo applications.

【 授权许可】

Unknown