Sensing of EGTA Mediated Barrier Tissue Disruption with an Organic Transistor

Biosensors

Scherrine Tria¹  Leslie H. Jimison¹  Adel Hama²  Manuelle Bongo² 

[1]Department of Bioelectronics, Ecole Nationale Supérieure des Mines, CMP-EMSE, MOC 880 Rue de Mimet, Gardanne 13541, France

关键词: organic bioelectronics;    tight junctions;    paracellular transport;    EGTA;    barrier tissue;    toxicology;    biosensing;    organic electrochemical transistor;   

DOI  :  10.3390/bios3010044

来源: mdpi

【 摘 要 】

Barrier tissue protects the body against external factors by restricting the passage of molecules. The gastrointestinal epithelium is an example of barrier tissue with the primary purpose of allowing the passage of ions and nutrients, while restricting the passage of pathogens and toxins. It is well known that the loss of barrier function can be instigated by a decrease in extracellular calcium levels, leading to changes in protein conformation and an increase in paracellular transport. In this study, ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetra acetic acid (EGTA), a calcium chelator, was used to disrupt the gastrointestinal epithelial barrier. The effect of EGTA on barrier tissue was monitored by a novel label-free method based on an organic electrochemical transistor (OECT) integrated with living cells and validated against conventional methods for measuring barrier tissue integrity. We demonstrate that the OECT can detect breaches in barrier tissue upon exposure to EGTA with the same sensitivity as existing methods but with increased temporal resolution. Due to the potential of low cost processing techniques and the flexibility in design associated with organic electronics, the OECT has great potential for high-throughput, disposable sensing and diagnostics.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.