Influence of Nivolumab for Intercellular Adhesion Force between a T Cell and a Cancer Cell Evaluated by AFM Force Spectroscopy

Sensors

Hyonchol Kim¹  Chikashi Nakamura¹  Shun’ichi Kuroda²  Masumi Iijima³  Kenta Ishibashi⁴ 

[1] Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba 305-8565, Japan;Department of Biomolecular Science and Reaction, The Institute of Scientific and Industrial Research, Osaka University, Osaka 567-0047, Japan;Department of Nutritional Science and Food Safety, Faculty of Applied Bioscience, Tokyo University of Agriculture, Tokyo, 156-8502, Japan;Graduate School of Engineering, Tokyo University of Agriculture and Technology, Tokyo 184-8588, Japan;

关键词: atomic force microscopy;    nivolumab;    PD-1;    PD-L1;    cell adhesion;    microcup;   

DOI  :  10.3390/s20195723

来源: DOAJ

【 摘 要 】

The influence of nivolumab on intercellular adhesion forces between T cells and cancer cells was evaluated quantitatively using atomic force microscopy (AFM). Two model T cells, one expressing high levels of programmed cell death protein 1 (PD-1) (PD-1^high Jurkat) and the other with low PD-1 expression levels (PD-1^low Jurkat), were analyzed. In addition, two model cancer cells, one expressing programmed death-ligand 1 (PD-L1) on the cell surface (PC-9, PD-L1⁺) and the other without PD-L1 (MCF-7, PD-L1⁻), were also used. A T cell was attached to the apex of the AFM cantilever using a cup-attached AFM chip, and the intercellular adhesion forces were measured. Although PD-1^high T cells adhered strongly to PD-L1⁺ cancer cells, the adhesion force was smaller than that with PD-L1⁻ cancer cells. After the treatment of PD-1^high T cells with nivolumab, the adhesion force with PD-L1⁺ cancer cells increased to a similar level as with PD-L1⁻ cancer cells. These results can be explained by nivolumab influencing the upregulation of the adhesion ability of PD-1^high T cells with PD-L1⁺ cancer cells. These results were obtained by measuring intercellular adhesion forces quantitatively, indicating the usefulness of single-cell AFM analysis.

【 授权许可】

Unknown