Evaluation of Antioxidant and Antibacterial Activities of Aqueous, Methanolic and Alkaloid Extracts from Mitragyna Speciosa (Rubiaceae Family) Leaves

Molecules

Suhanya Parthasarathy¹  Juzaili Bin Azizi¹  Surash Ramanathan¹  Sabariah Ismail¹  Sreenivasan Sasidharan¹  Mohd I. Mohd. Said¹ 

[1] ¹Centre for Drug Research, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia ²Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800 USM, Pulau Penang, Malaysia ³School of Chemical Sciences and Food Technology Universiti Kebangsaan Malaysia 43600 Bangi, Selangor, Malaysia

关键词: Mitragyna speciosa;    antioxidant;    antibacterial;   

DOI  :  10.3390/molecules14103964

来源: mdpi

【 摘 要 】

Studies on the antioxidant and antimicrobial activities of Mitragyna speciosa leaf extracts are lacking. In this study the antioxidant properties of water, methanolic and alkaloid M. speciosa leaf extracts were evaluated using the DPPH (2,2-diphenyl-1- picrylhydrazyl) radical scavenging method. The amount of total phenolics and flavanoid contents were also estimated. The DPPH IC₅₀ values of the aqueous, alkaloid and methanolic extracts were 213.4, 104.81 and 37.08 μg/mL, respectively. The total phenolic content of the aqueous, alkaloid and methanolic extracts were 66.0 mg, 88.4, 105.6 mg GAE/g, respectively, while the total flavanoid were 28.2, 20.0 and 91.1 mg CAE/g respectively. The antioxidant activities were correlated with the total phenolic content. This result suggests that the relatively high antioxidant activity of the methanolic extract compared to aqueous and alkaloid extract could be possibly be due to its high phenolic content. The aqueous, alkaloid and methanolic extracts were screened for antimicrobial activity. The extracts showed antimicrobial activity against Salmonella typhi and Bacillus subtilis. The minimum inhibitory concentrations (MICs) of extracts determined by the broth dilution method ranged from 3.12 to 6.25 mg/mL. The alkaloid extract was found to be most effective against all of the tested organisms.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.