Evaluation of cytotoxicity and biological activities in extracellular polysaccharides released by cyanobacterium Arthrospira platensis

Brazilian Archives of Biology and Technology

Rafika Challouf¹  Lamia Trabelsi¹  Rym Ben Dhieb¹  Omeya El Abed¹  Ali Yahia¹  Khamissa Ghozzi¹  Jihen Ben Ammar¹  Hela Omran¹  Hatem Ben Ouada¹ 

[1] ,Institut National des Sciences et Technologies de la Mer Laboratoire de Biodiversité et Biotechnologie Marine

关键词: Cyanobacteria;    Exoplysaccharides;    cytotoxicity;    antibacterial;    antioxidant;    activity;   

DOI  :  10.1590/S1516-89132011000400024

来源: SciELO

【 摘 要 】

The aim of this work was to evaluate the cytotoxicity of Arthrospira platensis Extracellular Polymeric Substances (EPS) for colon cancer and kidney cell lines. Results showed that EPS were free from cytotoxic effects. A variety of solvents were assessed for their ability to extract the bioactive ingredients from EPS. Methanol gave the highest yield (75.75%) than other solvents. The extracts were tested for activities against a collection of Gram+/- bacteria. The methanol extract exhibited a more potent activity than the other organic extracts, whereas the aqueous extract was active against Staphylococcus epidermis (Gram+) and Salmonella typhimurium (Gram-). Finally, The extracts were also tested for the antioxidant activity, using the Trolox Equivalent Antioxidant Activity assay. The methanol extract displayed a moderate antioxidant activity (TEAC = 0.027 mg/ml). The HPLC analysis of this extract revealed two distinct peaks: 8.1 kDa (8.31 min) and 4.1 kDa (8.54 min).

【 授权许可】

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