Trametes sp. as a Source of Biopolymer Cross-Linking Agents: Laccase Induced Gelation of Ferulated Arabinoxylans

Molecules

Alva Castillo¹  Agustín Rascón-Chu¹  Georgina Vargas¹  Elizabeth Carvajal-Millán¹  Elisa Valenzuela-Soto¹  Rogerio R. Sotelo-Mundo¹ 

[1]¹Facultad de Zootecnia, Universidad Autónoma de Chihuahua, Chihuahua, Chihuahua 31120, Mexico

关键词: polysaccharide;    cross-linking;    ferulic acid;    viscosity;    gels;   

DOI  :  10.3390/molecules14104159

来源: mdpi

【 摘 要 】

This study examined the feasibility of using Trametes sp. grown under drought conditions to catalyze the covalent cross-linking of ferulated arabinoxylans. The rate of polymerization of ferulated arabinoxylans solutions at 0.5% (w/v) was dose dependent on the laccase concentration in the system. Above 1.2 nkat laccase/mg ferulated arabinoxylan, the rate of cross-linking reached a plateau, suggesting that the reaction reached saturation. For 2% (w/v) ferulated arabinoxylans treated with laccase (1.6 nkat/mg ferulated arabinoxylan), stable gels were formed after 4 h at 25 °C, with cross-linking contents (diferulic and triferulic acid) contents of 0.03 and 0.015 μg/mg ferulated arabinoxylan, respectively. This study demonstrated that Trametes sp. can be a source of biopolymer cross-linking enzymes like laccase.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.