In Vitro Antioxidant Activities, Free Radical Scavenging Capacity, and Tyrosinase Inhibitory of Flavonoid Compounds and Ferulic Acid from Spiranthes sinensis (Pers.) Ames

Molecules

Chung Pin Liang¹  Chia Hao Chang²  Chien Cheng Liang⁴  Kuei Yu Hung³ 

[1]Cheng Ching General Hospital, Taichung 42881, Taiwan

[2] E-Mail:

[3]Department of Medicinal Botanicals and Health Applications, Da-Yeh University, Changhua 51591, Taiwan

[4] E-Mail:

[5]Pemay Biomedical Technology Corp, Taichung 41348, Taiwan

[6] E-Mail:

[7]Horien International Co., Ltd., Taichung 42881, Taiwan

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关键词: Spiranthes sinensis;    ultrasound-assisted extraction;    ferulic acid;    flavonoids;    antioxidant activity;    tyrosinase;   

DOI  :  10.3390/molecules19044681

来源: mdpi

【 摘 要 】

In this study, ultrasound-assisted extraction (UAE) and other methods of extracting flavonoid compounds and ferulic acid (FA) from S. sinensis were investigated. Five different extraction methods, including water extraction (W), water extraction using UAE (W+U), 75% ethanol extraction (E), 75% ethanol extraction using UAE (E+U), and supercritical CO₂ extraction (SFE) were applied in the extraction of bioactive compounds (flavonoids and ferulic acid) in order to compare their efficiency. The highest yield of flavonoids (4.28 mg/g) and ferulic acid (4.13 mg/g) content was detected in the E+U extract. Furthermore, S. sinensis extracts obtained by E+U show high antioxidant activity, and IC₅₀ values of 0.47 mg/mL for DPPH radicals and 0.205 mg/mL for metal chelating activity. The total antioxidant assay shows superoxide radical scavenging capacity and in vitro mushroom tyrosinase inhibition in a dose-dependent manner, suggesting that E+U can be used for extraction of bioactive compounds from S. sinensis.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.