Dominant-Negative Proteins in Herpesviruses – From Assigning Gene Function to Intracellular Immunization

Viruses

Hermine Mühlbach¹  Christian A. Mohr¹  Zsolt Ruzsics² 

[1] These authors contributed equally to this work.;>Max-von-Pettenkofer Institut, LMU, Feodor-Lynenstr. 25, 81377 Munich, Germany;E-Mails: [e-mail] (H.M.); [e-mail] (C.A.M.); [e-mail] (Z.R

关键词: dominant-negative;    essential genes;    random mutagenesis;    conditional gene expression;    deletion;    intracellular immunization;    herpesvirus;    conserved gene blocks;   

DOI  :  10.3390/v1030420

来源: mdpi

【 摘 要 】

Investigating and assigning gene functions of herpesviruses is a process, which profits from consistent technical innovation. Cloning of bacterial artificial chromosomes encoding herpesvirus genomes permits nearly unlimited possibilities in the construction of genetically modified viruses. Targeted or randomized screening approaches allow rapid identification of essential viral proteins. Nevertheless, mapping of essential genes reveals only limited insight into function. The usage of dominant-negative (DN) proteins has been the tool of choice to dissect functions of proteins during the viral life cycle. DN proteins also facilitate the analysis of host-virus interactions. Finally, DNs serve as starting-point for design of new antiviral strategies.

【 授权许可】

CC BY   
© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland