Regulation of Nodal Signalling and Mesendoderm Formation by TARAM-A, a TGFβ-Related Type I Receptor

Developmental Biology

Laure Saint-Etienne¹  Juliette Mathieu¹  Frédéric M. Rosa¹  Michael R. Rebagliati¹  Tazu O. Aoki¹  Nadine Peyriéras¹ 

[1] U368 INSERM, Ecole Normale Supérieure, 46 rue d'Ulm, 75230, Paris, France

关键词: zebrafish;    nodal;    endoderm;    signalling;    dominant-negative;   

DOI  :  10.1006/dbio.2001.0510

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Nodalsignallingisessentialformanydevelopmentaleventsduringvertebratedevelopment,includingtheestablishmentofleft–rightasymmetry,ofdorsoventralaxisofthecentralnervoussystem,andendodermandmesodermformation.ThezebrafishTGFβ-relatedtypeIreceptor,TARAM-A(Tar),isexpressedintheprospectivemesendodermalterritoryand,whenactivated,cantransfateearlyblastomeresintoendoderm,suggestingthatNodalandTarmayrepresentsimilarsignallingpathways.Wehaveanalysedthefunctionalrelationshipsbetweenthosetwopathwaysinzebrafish.Wefirstdemonstratethattarandthezebrafishnodalgenescycandsqtfunctionallyinteract.Wealsoshowthatadominant-negativeisoformofTar,TarMR,interferesspecificallywiththefunctionofCycandSqtinvitro,butdoesnotinterferewiththefunctionofBMP2,anotherTGFβ-relatedmolecule.TarMRinterferesalsowithNodalsignallinginvivosinceitenhancesthephenotypeofembryoswithweakenedNodalsignalling.OverexpressionoftarMRinwild-typeembryosinterferedwiththeformationofendoderm-derivedstructures.Conversely,overexpressionoftarenlargedthepresumptivemesendodermalregionattheonsetofgastrulation.Together,ourresultspointtoTarasanessentialfactorforendodermformationandanimportantmodulatorofNodalsignalling,potentiallyrepresentingoneoftheNodalreceptors.

【 授权许可】

Unknown