Host-Guest Complexation Studied by Fluorescence Correlation Spectroscopy: Adamantane–Cyclodextrin Inclusion

International Journal of Molecular Sciences

Daniel Granadero²  Jorge Bordello²  Maria Jesus Pérez-Alvite¹  Mercedes Novo² 

[1] Departamento de Química Orgánica, Facultade de Química, Universidade de Santiago de Compostela, E-15782 Santiago de Compostela, Spain; E-Mail:;Departamento de Química Física, Facultade de Ciencias, Universidade de Santiago de Compostela, E-27002 Lugo, Spain; E-Mails:

关键词: fluorescence correlation spectroscopy;    host-guest chemistry;    adamantane;    cyclodextrin;   

DOI  :  10.3390/ijms11010173

来源: mdpi

【 摘 要 】

The host-guest complexation between an Alexa 488 labelled adamantane derivative and β-cyclodextrin is studied by Fluorescence Correlation Spectroscopy (FCS). A 1:1 complex stoichiometry and a high association equilibrium constant of K = 5.2 × 10⁴ M⁻¹ are obtained in aqueous solution at 25 °C and pH = 6. The necessary experimental conditions are discussed. FCS proves to be an excellent method for the determination of stoichiometry and association equilibrium constant of this type of complexes, where both host and guest are nonfluorescent and which are therefore not easily amenable to standard fluorescence spectroscopic methods.

【 授权许可】

CC BY   
© 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.