Quercetin as a Potential Modulator of P-Glycoprotein Expression and Function in Cells of Human Pancreatic Carcinoma Line Resistant to Daunorubicin

Molecules

Sylwia Borska¹  Miroslaw Sopel¹  Magdalena Chmielewska¹  Maciej Zabel¹ 

[1] ¹Department of Histology and Embryology, Medical University, T. Chalubinski Street 6a, 50-368 Wroclaw, Poland ²Department of Histology and Embryology, University of Medical Sciences in Poznan, Swiecickiego Street 6, 60-781 Poznan, Poland ³Lower Silesian Centre of Oncology, Hirszfeld Square 12, 53-413 Wroclaw, Poland

关键词: quercetin;    daunorubicin;    P-glycoprotein;    multidrug resistance;   

DOI  :  10.3390/molecules15020857

来源: mdpi

【 摘 要 】

P-glycoprotein (P-gp) is one of the ABC transporters responsible for the resistance of several tumours to successful chemotherapy. Numerous agents are capable of interfering with the P-gp-mediated export of drugs but unfortunately most of them produce serious side effects. Some plant polyphenols, including the flavonol quercetin (Q), manifest anti-neoplastic activity mainly due to their influence on cell cycle control and apoptosis. Reports are also available which show that Q may intensify action of cytostatic drugs and suppress the multidrug resistance (MDR) phenomenon. The study aimed at determination if Q sensitizes cells resistant to daunorubicin (DB) through its effect on P-gp expression and action. The experiments were conducted on two cell lines of human pancreatic carcinoma, resistant to DB EPP85-181RDB and sensitive EPP85-181P as a comparison. Cells of both lines were exposed to selected concentrations of Q and DB, and then membranous expression of P-gp and its transport function were examined. The influence on expression of gene for P-gp (ABCB1) was also investigated. Results of the studies confirmed that Q affects expression and function of P-gp in a concentration-dependent manner. Moreover it decreased expression of ABCB1. Thus, Q may be considered as a potential modulator of P-gp.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.