Following Enzyme Activity with Infrared Spectroscopy

Sensors

Saroj Kumar¹ 

[1] Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden; E-Mail

关键词: vibrational spectroscopy;    infrared spectroscopy;    ATR;    FTIR;    enzyme activity;   

DOI  :  10.3390/s100402626

来源: mdpi

【 摘 要 】

Fourier transform infrared (FTIR) spectroscopy provides a direct, “on-line” monitor of enzymatic reactions. Measurement of enzymatic activity is based on the fact that the infrared spectra of reactants and products of an enzymatic reaction are usually different. Several examples are given using the enzymes pyruvate kinase, fumarase and alcohol dehydrogenase. The main advantage of the infrared method is that it observes the reaction of interest directly, i.e., no activity assay is required to convert the progress of the reaction into an observable quantity.

【 授权许可】

CC BY   
© 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.