Automation in the High-throughput Selection of Random Combinatorial Libraries—Different Approaches for Select Applications

Molecules

Jörn Glökler¹  Tatjana Schütze¹ 

[1]¹Department of Vertebrate Genomics, Max Planck Institute for Molecular Genetics, Ihnestr. 63-73, 14195 Berlin, Germany[2] height:6px

[3]"> ²Institute for Chemistry/ Biochemistry, Free University Berlin, Thielallee 63, 14195 Berlin, Germany

关键词: phage display;    SELEX;    automation;    random combinatorial library;    in vitro evolution;   

DOI  :  10.3390/molecules15042478

来源: mdpi

【 摘 要 】

Automation in combination with high throughput screening methods has revolutionised molecular biology in the last two decades. Today, many combinatorial libraries as well as several systems for automation are available. Depending on scope, budget and time, a different combination of library and experimental handling might be most effective. In this review we will discuss several concepts of combinatorial libraries and provide information as what to expect from these depending on the given context.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.