Effects of Aflatoxin B1 and Fumonisin B1 on the Viability and Induction of Apoptosis in Rat Primary Hepatocytes

International Journal of Molecular Sciences

Deise H. B. Ribeiro¹  Fabiane L. Ferreira²  Valéria N. da Silva²  Simone Aquino¹ 

[1] Centro de P&D de Proteção Ambiental, Instituto Biológico, Av. Conselheiro Rodrigues Alves n. 1252 – Vila Mariana. São Paulo, SP. 04014-002, Brazil;Departamento de Microbiologia, Instituto de Ciências Biomédicas – Universidade de São Paulo, Av. Prof. Lineu Prestes n. 1374 – Cidade Universitária. São Paulo, SP. 05508-900, Brazil

关键词: aflatoxin B₁;    apoptosis;    fumonisin B₁;    primary hepatocytes;   

DOI  :  10.3390/ijms11041944

来源: mdpi

【 摘 要 】

The present study evaluated the effect of aflatoxin B₁ (AFB₁) and fumonisin B₁ (FB₁) either alone, or in association, on rat primary hepatocyte cultures. Cell viability was assessed by flow cytometry after propidium iodine intercalation. DNA fragmentation and apoptosis were assessed by agarose gel electrophoresis and acridine orange and ethidium bromide staining. At the concentrations of AFB₁ and FB₁ used, the toxins did not decrease cell viability, but did induce apoptosis in a concentration and time-dependent manner.

【 授权许可】

CC BY   
© 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.