The Individual and Combined Effects of Deoxynivalenol and Aflatoxin B1 on Primary Hepatocytes of Cyprinus Carpio

International Journal of Molecular Sciences

Cheng-Hua He¹  Yan-Hong Fan²  Ying Wang¹  Chao-Ying Huang¹  Xi-Chun Wang¹ 

[1] College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China; E-Mails:;Wujiang agricultural commission, Wujiang, 215200, China; E-Mail:

关键词: deoxynivalenol;    aflatoxin B₁;    cytotoxicity;    Cyprinus carpio;    primary hepatocytes;   

DOI  :  10.3390/ijms11103760

来源: mdpi

【 摘 要 】

Aflatoxin B₁ (AFB₁) and deoxynivalenol (DON) are important food-borne mycotoxins that have been implicated in animal and human health. In this study, individual and combinative effects of AFB₁ and DON were tested in primary hepatocytes of Cyprinus carpio. The results indicated that the combinative effects of AFB₁ and DON (0.01 μg/mL AFB₁ and 0.25 μg/mL DON; 0.02 μg/mL AFB₁ and 0.25 μg/mL DON; 0.02 μg/mL AFB₁ and 0.5 μg/mL DON) were higher than that of individual mycotoxin (P < 0.05). The activity of AST, ALT and LDH in cell supernatant was higher than that of control group (P < 0.05) when the mycotoxins were exposed to primary hepatocytes for 4 h. The decreased cell number was observed in tested group by inverted light microscopy. The mitochondrial swelling, endoplasmic reticulum dilation and a lot of lipid droplets were observed in primary hepatocytes by transmission electron microscope. Therefore, this combination was classified as an additive response of the two mycotoxins.

【 授权许可】

CC BY   
© 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.