Label Free Detection of CD4+ and CD8+ T Cells Using the Optofluidic Ring Resonator

Sensors

John T. Gohring¹ 

[1] Department of Biological Engineering, 240D Bond Life Sciences Center, 1201 East Rollins Street, University of Missouri, Columbia, MO 65211, USA; E-Mail:

关键词: HIV;    CD4;    CD8;    ring resonator;    optical biosensor;    label free;   

DOI  :  10.3390/s100605798

来源: mdpi

【 摘 要 】

We have demonstrated label free detection of CD4+ and CD8+ T-Lymphocyte whole cells and CD4+ T-Lymphocyte cell lysis using the optofluidic ring resonator (OFRR) sensor. The OFRR sensing platform incorporates microfluidics and photonics in a setup that utilizes small sample volume and achieves a fast detection time. In this work, white blood cells were isolated from healthy blood and the concentrations were adjusted to match T-Lymphocyte levels of individuals infected with HIV. Detection was accomplished by immobilizing CD4 and CD8 antibodies on the inner surface of the OFRR. Sensing results show excellent detection of CD4+ and CD8+ T-Lymphocyte cells at medically significant concentrations with a detection time of approximately 30 minutes. This work will lead to a rapid and low-cost sensing device that can provide a CD4 and CD8 count as a measure of HIV progression.

【 授权许可】

CC BY   
© 2010 by the authors; licensee MDPI, Basel, Switzerland.