Biocatalysts Immobilized in Ultrathin Ordered Films

Sensors

Jadwiga Sołoducho¹ 

关键词: biosensors;    enzyme immobilization;    Langmuir-Blodgett technique;    enzyme activity;    AFM;   

DOI  :  10.3390/s101110298

来源: mdpi

【 摘 要 】

The immobilization of enzymes and other proteins into ordered thin materials has attracted considerable attention over the past few years. This research has demonstrated that biomolecules immobilized in different [Langmuir-Blodgett (LB)/Langmuir-Schaefer (LS)] matrixes retain their functional characteristics to a large extent. These new materials are of interest for applications as biosensors and biocatalysts. We review the growing field of oxidases immobilized onto ordered Langmiur-Blodgett and Langmuir-Schaefer films. Strategies for the preparation of solid supports and the essential properties of the resulting materials with respect to the envisaged applications are presented. Basic effects of the nature of the adsorption and various aspects of the application of these materials as biosensors, biocatalysts are discussed. Outlook of potential applications and further challenges are also provided.

【 授权许可】

CC BY   
© 2010 by the authors; licensee MDPI, Basel, Switzerland.