Enzymatic activity of catechol 1,2-dioxygenase and catechol 2,3-dioxygenase produced by Gordonia polyisoprenivorans

Química Nova

Andréa Scaramal Silva²  Flávio Anastácio De Oliveira Camargo²  Robson Andreazza²  Rodrigo Josemar Seminoti Jacques¹  Daiana Bortoluzzi Baldoni¹  Fátima M. Bento¹ 

[1] ,Universidade Federal do Rio Grande do Sul Departamento de Ciência do Solo Porto Alegre RS ,Brasil

关键词: anthracene;    enzyme activity;    enzyme immobilization;   

DOI  :  10.1590/S0100-40422012000800018

来源: SciELO

【 摘 要 】

This study aimed to evaluate the environmental conditions for enzyme activity of catechol 1,2-dioxygenase (C1,2O) and catechol 2,3-dioxygenase (C2,3O) produced by Gordonia polyisoprenivorans in cell-free and immobilized extracts. The optimum conditions of pH, temperature, time course and effect of ions for enzyme activity were determined. Peak activity of C1,2O occurred at pH 8.0. The isolate exhibited the highest activity of C2,3O at pH 7.0 and 8.0 for the cell-free extract and immobilized extract, respectively. This isolate exhibited important characteristics such as broad range of pH, temperature and time course for enzyme activity.

【 授权许可】

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