A Simple and Rapid Method for DNA Isolation from Xylophagous Insects

International Journal of Molecular Sciences

Nancy Calderón-Cortés²  Mauricio Quesada²  Horacio Cano-Camacho¹ 

[1] Multidisciplinary Center of Biotechnology Studies (CMEB), Michoacan University of San Nicolas of Hidalgo (UMSNH), Morelia, Mexico; E-Mails:;Center for Ecosystems Research (CIEco), National Autonomous University of Mexico (UNAM), Morelia, Mexico; E-Mail:

关键词: Cerambycidae;    DNA isolation;    PCR;    Oncideres albomarginata chamela;    phenolics;    restriction digestion;    xylophagous insects;   

DOI  :  10.3390/ijms11125056

来源: mdpi

【 摘 要 】

Published methods to isolate DNA from insects are not always effective in xylophagous insects because they have high concentrations of phenolics and other secondary plant compounds in their digestive tracts. A simple, reliable and labor-effective cetyltrimethylammonium bromide-polyvinylpyrrolidone (CTAB-PVP) method for isolation of high quality DNA from xylophagous insects is described. This method was successfully applied to PCR and restriction analysis, indicating removal of common inhibitors. DNA isolated by the CTAB-PVP method could be used in most molecular analyses.

【 授权许可】

CC BY   
© 2010 by the authors; licensee MDPI, Basel, Switzerland.