Antimicrobial Activity of Diterpenes from Viguiera arenaria against Endodontic Bacteria

Molecules

Tatiane C. Carvalho¹  Marília R. Simão¹  Sérgio R. Ambrósio¹  Niege A. J. C. Furtado¹  Rodrigo C. S. Veneziani¹  Vladimir C. G. Heleno¹  Fernando B. Da Costa¹  Brenda P. F. A. Gomes¹  Maria Gorete M. Souza¹  Erika Borges dos Reis¹ 

[1] ¹Laboratório de Pesquisa em Microbiologia Aplicada, Núcleo de Pesquisa em Ciências Exatas e Tecnológicas, Universidade de Franca, Franca, SP, Brazil

关键词: antimicrobial activity;    endodontic bacteria;    pimarane diterpenes;    structural requirements;    Viguiera arenaria;   

DOI  :  10.3390/molecules160100543

来源: mdpi

【 摘 要 】

Six pimarane-type diterpenes isolated from Viguiera arenaria Baker and two semi-synthetic derivatives were evaluated in vitro against a panel of representative microorganisms responsible for dental root canal infections. The microdilution method was used for the determination of the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against Porphyromonas gingivalis, Prevotella nigrescens, Prevotella intermedia, Prevotella buccae, Fusobacterium nucleatum, Bacteroides fragilis, Actinomyces naeslundii, Actinomyces viscosus, Peptostreptococcus micros, Enterococcus faecalis and Aggregatibacter actinomycetemcomitans. The compounds ent-pimara-8(14),15-dien-19-oic acid, its sodium salt and ent-8(14),15-pimaradien-3β-ol were the most active, displaying MIC values ranging from 1 to 10 μg mL^-1. The results also allow us to conclude that minor structural differences among these diterpenes significantly influence their antimicrobial activity, bringing new perspectives to the discovery of new chemicals for use as a complement to instrumental endodontic procedures.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.