N-Acetyl Glucosamine Obtained from Chitin by Chitin Degrading Factors in Chitinbacter tainanesis

International Journal of Molecular Sciences

Jeen-Kuan Chen²  Chia-Rui Shen³  Chao-Hsien Yeh¹  Bing-Shiun Fang²  Tung-Li Huang² 

[1] Department of Chemical Engineering, Mingchi University of Technology, Taishan, New Taipei, Taiwan; E-Mail:;Environment and Biotechnology Department, Refining and Manufacturing Research Institute, CPC Corporation, Taiwan, Chia-yi, Taiwan; E-Mail:;Department of Medical Biotechnology and Laboratory Science, Chang Gung University, Kweishan, Taoyuan, Taiwan; E-Mail:

关键词: CDFs;    Chitinibacter tainanensis;    chitin;    NAG;    fermentation;   

DOI  :  10.3390/ijms12021187

来源: mdpi

【 摘 要 】

A novel chitin-degrading aerobe, Chitinibacter tainanensis, was isolated from a soil sample from southern Taiwan, and was proved to produce N-acetyl glucosamine (NAG). Chitin degrading factors (CDFs) were proposed to be the critical factors to degrade chitin in this work. When C. tainanensis was incubated with chitin, CDFs were induced and chitin was converted to NAG. CDFs were found to be located on the surface of C. tainanensis. N-Acetylglucosaminidase (NAGase) and endochitinase activities were found in the debris, and the activity of NAGase was much higher than that of endochitinase. The optimum pH of the enzymatic activity was about 7.0, while that of NAG production by the debris was 5.3. These results suggested that some factors in the debris, in addition to NAGase and endochitinase, were crucial for chitin degradation.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.