High Affinity, Developability and Functional Size: The Holy Grail of Combinatorial Antibody Library Generation

Molecules

Dirk Ponsel¹  Julia Neugebauer¹  Kathrin Ladetzki-Baehs¹ 

[1] MorphoSys AG, Lena-Christ Strasse 48, 82152 Martinsried, Germany

关键词: phage display;    library  ;   

DOI  :  10.3390/molecules16053675

来源: mdpi

【 摘 要 】

Since the initial description of phage display technology for the generation of human antibodies, a variety of selection methods has been developed. The most critical parameter for all in vitro-based approaches is the quality of the antibody library. Concurrent evolution of the libraries has allowed display and selection technologies to reveal their full potential. They come in different flavors, from naïve to fully synthetic and differ in terms of size, quality, method of preparation, framework and CDR composition. Early on, the focus has mainly been on affinities and thus on library size and diversity. Subsequently, the increased awareness of developability and cost of goods as important success factors has spurred efforts to generate libraries with improved biophysical properties and favorable production characteristics. More recently a major focus on reduction of unwanted side effects through reduced immunogenicity and improved overall biophysical behavior has led to a re-evaluation of library design.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.