Phage Display for the Generation of Antibodies for Proteome Research, Diagnostics and Therapy

Molecules

Thomas Schirrmann¹  Torsten Meyer¹  Mark Schütte¹  André Frenzel¹ 

[1] Technische Universität Braunschweig, Institute of Biochemistry and Biotechnology, Department of Biotechnology, Spielmannstr. 7, 38106 Braunschweig, Germany ^†Present address: Merck KGaA, Frankfurter Str. 250, 64293 Darmstadt, Germany

关键词: antibody;    scFv;    phage display;    cancer;    diagnostics;    proteome research;    panning;   

DOI  :  10.3390/molecules16010412

来源: mdpi

【 摘 要 】

Twenty years after its development, antibody phage display using filamentous bacteriophage represents the most successful in vitro antibody selection technology. Initially, its development was encouraged by the unique possibility of directly generating recombinant human antibodies for therapy. Today, antibody phage display has been developed as a robust technology offering great potential for automation. Generation of monospecific binders provides a valuable tool for proteome research, leading to highly enhanced throughput and reduced costs. This review presents the phage display technology, application areas of antibodies in research, diagnostics and therapy and the use of antibody phage display for these applications.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.