Lichtheimia blakesleeana as a New Potencial Producer of Phytase and Xylanase

Molecules

Maria Luiza Carvalho Neves¹  Milena Fernandes da Silva¹  Cristina Maria Souza-Motta¹  Michele Rigon Spier¹  Carlos Ricardo Soccol¹  Tatiana Souza Porto¹  Keila Aparecida Moreira¹ 

[1] ¹Renorbio/ UECE, Av. Paranjana, 1.700, 60740-000, Fortaleza, Ceará, Brazil

关键词: phytase;    xylanase;    citrus pulp;    solid-state fermentation;    Lichtheimia blakesleeana;   

DOI  :  10.3390/molecules16064807

来源: mdpi

【 摘 要 】

Brazil is known for its great potential for production of renewable resources such as agro-industrial residues. These residues can be used as alternative sources of new products. Meanwhile, solid-state fermentation, with its advantages of energy conservation and pollution reduction, has been identified as a process of great potential for the production of bioactive compounds, especially enzymes. In the present work, a 2³ factorial design was used to evaluate the effects of pH, temperature and moisture on the production of phytase and xylanase by Lichtheimia blakesleeana URM 5604 through the fermentation of citrus pulp. Statistical analyses of the results showed that the only the pH influenced the production of these enzymes, with the best phytase production (264.68 U/g) ocurring at pH 6.0, 34 °C, initial moisture 50%, after 48 hours of culture. The best conditions for xylanase production (397.82 U/g) were fermentation for 120 hours at pH 4.0, 26 °C and initial moisture of 70%. The best parameters for the simultaneous production of phytase (226.92 U/g) and xylanase (215.59 U/g) were determined to be initial moisture of 50%, pH 6.0, 26 °C, and 48 hours of fermentation.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.