Generation and Detection of Levuglandins and Isolevuglandins In Vitro and In Vivo

Molecules

Ming Zhang¹  Wei Li¹ 

[1] ¹Reproductive Medicine Center, Zhongnan Hospital of Wuhan University, Wuhan 430071, China ²Office of the Texas State Chemist, Texas A&M University, College Station, TX 77845, USA ³Department of Ophthalmology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China

关键词: levuglandin;    polyunsaturated fatty acids;    oxidative injury;   

DOI  :  10.3390/molecules16075333

来源: mdpi

【 摘 要 】

Levuglandins (LGs) and isolevuglandins (isoLGs), formed by rearrangement of endoperoxide intermediates generated through the cyclooxygenase and free radical induced oxidation of polyunsaturated fatty acids (PUFAs), are extraordinarily reactive, forming covalent adducts incorporating protein lysyl e-amino groups. Because they accumulate, these adducts provide a dosimeter of oxidative injury. This review provides an updated and comprehensive overview of the generation of LG/isoLG in vitro and in vivo and the detection methods for the adducts of LG/isoLG and biological molecules in vivo.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.