Assessment of Bioflocculant Production by Bacillus sp. Gilbert, a Marine Bacterium Isolated from the Bottom Sediment of Algoa Bay

Marine Drugs

Piyo Nontembiso¹  Cosa Sekelwa¹  Mabinya V. Leonard¹ 

[1] Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa;

关键词: marine;    Algoa bay;    Bacillus sp. Gilbert;    bioflocculant;    polysaccharide;   

DOI  :  10.3390/md9071232

来源: mdpi

【 摘 要 】

The bioflocculant-producing potentials of a marine bacteria isolated from the bottom sediment of Algoa Bay was investigated using standard methods. The 16S rDNA sequence analysis revealed 98% similarity to that of Bacillus sp. HXG-C1 and the nucleotide sequence was deposited in GenBank as Bacillus sp. Gilbert with accession number HQ537128. Bioflocculant was optimally produced when sucrose (72% flocculating activity) and ammonium chloride (91% flocculating activity) were used as sole sources of carbon and nitrogen, respectively; an initial pH 6.2 of the production medium; and Mg²⁺ as cation. Chemical analysis of the purified bioflocculant revealed the compound to be a polysaccharide.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland